Journal: Journal of Biological Chemistry

Article Title: Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model

doi: 10.1016/j.jbc.2021.100482

Figure Lengend Snippet: Figure 1. Conditional knockouts of Exoc5 in mouse skeletal muscle were generated. A, fluorescent microscopy evaluating tissue-specific Cre expression using a mouse line with a tdTomato (To) reporter allele. The tissue sections are from female mice. B, recombination PCRs from genomic DNA of various tissues of tamoxifen-treated male mice to detect deletion of exons 7 to 10. C, real-time quantitative PCR measuring skeletal-muscle-specific EXOC5 mRNA expression (normalized to RPLP0) in Exoc5-SMKO and CTRL mice. D, representative image of a Western blot analysis (upper panel) used for the quantification (lower panel) of EXOC5 protein levels (normalized to Vinculin) in the soleus of Exoc5-SMKO and CTRL mice. Error bars = standard deviation, *p ≤0.05, **p ≤0.01.

Article Snippet: Chem. (2021) 296 100482 After permeabilization and blocking, sections were incubated with primary antibodies (anti-insulin (Cat #66198-1-Ig, Proteintech Group, Inc); anti-glucagon (Cat # 15954-1- AP,Proteintech Group, Inc)) overnight at 4 C and incubated with appropriate fluorescent secondary antibodies (DyLight 488 Anti-Rabbit IgG and DyLight 594 Anti-Mouse, Vector Laboratories) for detection.

Techniques: Generated, Microscopy, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal: Cell reports

Article Title: Boost immunizations with NA-derived peptide conjugates achieve induction of NA inhibition antibodies and heterologous influenza protections.

doi: 10.1016/j.celrep.2023.112766

Figure Lengend Snippet: Figure 3. Titration of rtN1- and rtN2-spe- cific serum IgG in ELISA The sera samples collected 2 weeks after (A and D) prime immunization, (B and E) first booster, and (C and F) second booster were titrated for (A–C) rtN1 and (D–F) rtN2. Each dot represents the serum antibody titer from one mouse (n = 5 per group). Bars represent the mean values of serum antibody titers. The dash line represents the limit of detection, corresponding to the initial serum dilution. The positive wells were determined as OD values above mean +2 3 SD of the OD value of the initial pre-immune sera dilution. Statistical signifi- cance was analyzed by t test for (B), (C), (E), and (F). *p < 0.05, **p < 0.01, and ****p < 0.001. All experiments were repeated twice using the same serum samples and showed similar results.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-63His tag Sino Biological Cat#105327-MM02T-H Rabbit polyclonal anti-N1 Sino Biological Cat#11058-R001 Rabbit polyclonal anti-N2 Sino Biological Cat#40017-RP01 HRP-conjugated goat anti-human IgG Proteintech Group Cat#SA00001-17 HRP-conjugated goat anti-mouse IgG Proteintech Group Cat#SA00001-1 HRP-conjugated goat anti-mouse IgG1 Proteintech Group Cat#SA00012-1 HRP-conjugated goat anti-mouse IgG2a Proteintech Group Cat#SA00012-2 HRP-conjugated goat anti-mouse IgG3 Proteintech Group Cat#SA00012-5 HRP-conjugated goat anti-mouse IgM Proteintech Group Cat#SA00012-6 HRP-conjugated peanut agglutinin Sigma-Aldrich Cat#L7759 Bacterial and virus strains E.coli DH5a competent cells Lab stock N/A E.coli DH10Bac competent cells Lab stock N/A A/Hong Kong/8/1968 (H3N2) ATCC Cat#VR544TM A/New Jersey/8/1976 (H1N1) ATCC Cat#VR897TM A/Puerto Rico/8/1934 (H1N1) Haiyan Chang, Hunan Normal University N/A A/reassortant/NYMC X179A (H1N1) Haiyan Chang, Hunan Normal University N/A A/Guizhou/54/1989 (GZ89, H3N2) Haiyan Chang, Hunan Normal University N/A A/Puerto Rico/8/1934 (H1N1) Haiyan Chang, Hunan Normal University N/A Biological samples Human convalescent sera samples Yao-Qing Chen’s laboratory stock N/A Chemicals, peptides, and recombinant proteins N1P1 Top-peptide N/A N1P2 Top-peptide N/A N1P3 Top-peptide N/A N1P4 Top-peptide N/A N2P1 Top-peptide N/A N2P2 Top-peptide N/A N2P3 Top-peptide N/A N2P4 Top-peptide N/A rtN1 This paper N/A rtN2 This paper N/A KLH Solarbio Cat#K8160 Receptor destroying enzyme Denka Seiken Cat#340122 Fetuin from fetal bovine serum Sigma-Aldrich Cat#F2379 Incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Critical commercial assays ReadiLinkTM KLH Conjugation Kit AAT Bioquest Cat#5502 Mouse IFNg ELISPOT Kit BD Biosciences Cat#551083 Mouse IL-4 ELISPOT Kit Dakewe Cat#DKW22-2040-500 (Continued on next page) Cell Reports 42, 112766, July 25, 2023 11

Techniques: Titration, Enzyme-linked Immunosorbent Assay